The transmission uses coolers so not sure how that would play into this situation. Furthermore, to properly read the level, the VSA system has to be disable and the transmission shifted manually while the wheel are off the ground. The vehicle has to be idling too.Hypothetical situation, not a real one so don't freak out:
If the ambient temperature of my garage was 104*F and my new ATF fluid and my MDX had been parked in it for several days, one could assume all the surfaces, parts, and fluids in the vehicle had settled at the 104* ambient temperature. Then would it be fine to swap fluid and check the level plug?